We are delighted to introduce the "Textbook of Microbiology" designed specifically for B.Sc. semester II, aligning with the university curriculum. Although similar information can be found in conventional textbooks and online platforms, this book offers a fresh approach, featuring high-quality diagrams and images. The authors have leveraged their vast teaching experience and have diligently researched various esteemed resources, including international textbooks, library collections, and online databases. This text is not merely a compilation; it is a meticulously developed guide, complete with comprehensive explanations and accurately labeled illustrations. While some readers may encounter challenging terminology, the text ensures scientific precision. We encourage feedback and suggestions from our audience to enhance the book further.