This practical book aims to introduce Microbiology and Biochemistry to undergraduate and postgraduate students in Indian universities. The book "Lab Manual of Microbiology and Its Biochemistry," covers various sub-disciplines of microbiology and biotechnology. With over 100 experiments, the book focuses on both general and applied aspects, explained in a lucid and illustrative manner. Unique chapters not easily found elsewhere have been included for student convenience. Each experiment provides a detailed procedure for data recording in a practical notebook, accompanied by clear and simplified diagrams. The book is firmly rooted in scientific research and teaching. It emphasizes essential concepts, using straightforward language to highlight key aspects. Every experiment is structured with separate sections, including requirements, procedure, and results. Feedback and criticism from readers are highly valued, as they contribute to the ongoing improvement of this valuable resource.